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After that time, biofilms were washed with 200 l of PBS and incubated for 30 min at 37C with MitoTracker Green TM dye (Invitrogen) at final concentration of 500 nM, used to detect the change in total mitochondrial mass, and MitoTracker Orange (500 nM), used to detect the change in mitochondrial activity

If you spend a lot of time outdoors, reapply sunscreen to protect your skin from UV damage